منابع مشابه
DNA polymorphism detectable by restriction endonucleases.
Data on DNA polymorphisms detected by restriction endonucleases are rapidly accumulating. With the aim of analyzing these data, several different measures of nucleon (DNA segment) diversity within and between populations are proposed, and statistical methods for estimating these quantities are developed. These statistical methods are applicable to both nuclear and nonnuclear DNAs. When evolutio...
متن کاملDigestion of DNA with restriction endonucleases.
Restriction endonucleases recognize short DNA sequences and cleave double-stranded DNA at specific sites within or adjacent to the recognition sequences. Restriction endonuclease cleavage of DNA into discrete fragments is one of the most basic procedures in molecular biology. The first method presented in this unit is the cleavage of a single DNA sample with a single restriction endonuclease. A...
متن کاملOvalbumin gene. Action of restriction endonucleases upon DNA coding sequence.
A full length, double-stranded ovalbumin complementary DNA (cDNA) was synthesized in vitro and used to assay the action of 35 restriction endonucleases upon this DNA sequence. Some 22 enzymes, Bal I, BamHl, Blu I and its isoschizomerXho I, Ppu 1,Xba I, Tag I, Sal I, Bgl I, Bgl II, Kpn I, Sst II, Hue II, Hpa I, Hap II, Hha I, Hind11 and its isoschizomer HincII, Hind111 and its isoschizomer Hsu I...
متن کاملBlock of cleavage of ultraviolet-irradiated DNA with restriction endonucleases.
pBR322/Ultraviolet irradiation/Restriction endonuclease/Pyrimidine dimer Plasmid pBR322 DNA was irradiated with ultraviolet light at 254 nm and digested with various restric tion endonucleases. Analyses of the products suggested that the pyrimidine dimers which would have been formed just across the cutting site (Acc I), at the opposite position of the cutting site (Hind III, Eco RI, Bam HI), a...
متن کاملSelective Microbial Genomic DNA Isolation Using Restriction Endonucleases
To improve the metagenomic analysis of complex microbiomes, we have repurposed restriction endonucleases as methyl specific DNA binding proteins. As an example, we use DpnI immobilized on magnetic beads. The ten minute extraction technique allows specific binding of genomes containing the DpnI Gm6ATC motif common in the genomic DNA of many bacteria including γ-proteobacteria. Using synthetic ge...
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ژورنال
عنوان ژورنال: Acta Crystallographica Section A Foundations of Crystallography
سال: 2009
ISSN: 0108-7673
DOI: 10.1107/s0108767309096901